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anti dkk1  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti dkk1
    Anti Dkk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+dkk1/DKK1+Rabbit+mAb/pmc12686823-54-15-47
    Average 93 stars, based on 20 article reviews
    anti dkk1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway
    Article Snippet: .. Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. ..

    Reverse Transcription:

    Article Title: Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway
    Article Snippet: .. Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis.
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway
    Article Snippet: .. Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis.
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Microscopy:

    Article Title: Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway
    Article Snippet: .. Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. ..

    Cell Culture:

    Article Title: Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway
    Article Snippet: .. Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. ..

    Polymerase Chain Reaction:

    Article Title: Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway
    Article Snippet: .. Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis.
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Imaging:

    Article Title: Inhibitory effect of icariin on osteosarcoma cell proliferation via the Wnt/β-catenin signaling pathway
    Article Snippet: .. Osteosarcoma cancer cell line 143B (Cell Bank of the Chinese Academy of Sciences, Shanghai, China), methyl thiazolyl tetrazolium (MTT) and DMSO (both from Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), ICA (Aladdin Shanghai Biochemical Technology Co., Ltd., Shanghai, China), TRIzol kit and reverse transcription kit (both from Invitrogen, Carlsbad, CA, USA), VEGA ELISA kit and MMP-9 ELISA kit (both from R&D Systems, Inc., Minneapolis, MN, USA), rabbit anti-DKK1, rabbit anti-caspase-3, rabbit anti-β-catenin, rabbit anti-GSK3β, rabbit anti-pGSK3β (Ser9) and GAPDH (all from Cell Signaling Technology, Inc., Danvers, MA, USA), ECI luminescent solution (Invitrogen), inversed fluorescent microscope (Thermo Fisher Scientific GmbH, Dreieich, Germany), cell culture flask (Corning Inc., Corning, NY, USA), pipettor (Eppendorf, Hamburg, Germany), PCR instrument (Applied Biosystems, Foster City, CA, USA), UV imaging system (Biometra, Goettingen, Germany), and electronic balance (BP121S; Sartorius AG, Goettingen, Germany) were used in the study. ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis.
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Electrochemiluminescence:

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis.
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Nucleic Acid Electrophoresis:

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis.
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..

    Article Title: The role of Wnt signaling pathway in atherosclerosis and its relationship with angiogenesis
    Article Snippet: .. TRIzol kits, reverse transcription kits, electrochemiluminescence (ECL) solution (all from Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), enzyme-linked immunosorbent assay-interleukin-6 (ELISA-IL-6) kits, ELISA-tumor necrosis factor-α (TNF-α) kits (Wuhan Boster Biological Technology, Ltd., Wuhan, China), rabbit anti-Wnt1, rabbit anti-β-catenin, rabbit anti-DKK1, rabbit anti-VEGF, rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), horseradish peroxidase-labeled anti-rabbit secondary antibody (all from Cell Signaling Technology, Inc., Danvers, MA, USA), polymerase chain reaction (PCR) instrument, gel electrophoresis and transmembrane systems (both from Applied Biosystems; Thermo Fisher Scientific, Inc.), pipettors (Gilson, Inc., Middleton, WI, USA), ultraviolet imaging system (Biometra GmbH, Göttingen, Germany), fully automatic biochemical analyser (Toshiba, Tokyo, Japan) and electronic scales (BP121S; Sartorius AG, Göttingen, Germany). ..



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    Image Search Results


    Journal: Cell reports

    Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

    doi: 10.1016/j.celrep.2024.114527

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal anti-DKK1 (D5V6L) , Cell Signaling Technology , Cat# 48367S; RRID: AB_2799337.

    Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

    Journal: Cell reports

    Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

    doi: 10.1016/j.celrep.2024.114527

    Figure Lengend Snippet:

    Article Snippet: Rabbit monoclonal anti-DKK1 (1:100, Cell Signaling Technology, Cat# 48367S), goat polyclonal anti-SPARC (1:100, R&D Systems, Cat# AF941), mouse monoclonal anti-TIMP2 (1:100, R&D Systems, Cat# MAB971), goat polyclonal anti-β-catenin (1:100, R&D Systems, Cat# AF1329), goat polyclonal anti-integrin β1 (1:100, R&D Systems, Cat# AF1778), mouse monoclonal anti-COL1 (1:100, Cell Signaling Technology, Cat# 66948S), and phalloidin-iFluor 488 reagent (1:1000, Abcam, Cat# ab176753) were used.

    Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

    Characterization of organoid cultures and age differences in IESC phenotype: Organoid cultures derived from adult female (a-c) and male rats (d-f). Representative bright field images (n = 6) of IESC, plated in matrigel and cultured for 4–6 days (a,d). Scale bar: 25 μm. Immunohistochemistry (n = 6) for the intestinal stem cell marker Lgr5+, the epithelial cell marker NaKATPAse-α, and the cellular proliferation marker Ki67 all at 6 div in females (b,c) and males (e,f). Scale bar: 22 μm. Representative images (n = 5) for the Wnt signaling marker, Wnt3a (g) and its inhibitor, DKK1 (h), and the senescence marker γH2AX (i) in organoids derived from adult and middle-aged males and females. Scale bar: 32 μm. Histogram depicting mean (±SEM) proportion of cells positive for Wnt3a (n = 5) (j), DKK1 (n = 5) (k) and γH2AX (n = 5) (l). Two-way ANOVA followed by Tukey-multiple comparisons test. *: p < 0.05.

    Journal: Brain, behavior, and immunity

    Article Title: Intestinal epithelial stem cell transplants as a novel therapy for cerebrovascular stroke

    doi: 10.1016/j.bbi.2022.10.015

    Figure Lengend Snippet: Characterization of organoid cultures and age differences in IESC phenotype: Organoid cultures derived from adult female (a-c) and male rats (d-f). Representative bright field images (n = 6) of IESC, plated in matrigel and cultured for 4–6 days (a,d). Scale bar: 25 μm. Immunohistochemistry (n = 6) for the intestinal stem cell marker Lgr5+, the epithelial cell marker NaKATPAse-α, and the cellular proliferation marker Ki67 all at 6 div in females (b,c) and males (e,f). Scale bar: 22 μm. Representative images (n = 5) for the Wnt signaling marker, Wnt3a (g) and its inhibitor, DKK1 (h), and the senescence marker γH2AX (i) in organoids derived from adult and middle-aged males and females. Scale bar: 32 μm. Histogram depicting mean (±SEM) proportion of cells positive for Wnt3a (n = 5) (j), DKK1 (n = 5) (k) and γH2AX (n = 5) (l). Two-way ANOVA followed by Tukey-multiple comparisons test. *: p < 0.05.

    Article Snippet: Sections were incubated overnight at room temperature with primary antibodies (Lgr5+ (anti-rabbit)- 1:500ul, Novus Biological sciences, NLS1236; NaK-ATPase-alpha (anti-mouse)- 1: 500ul, Millipore, 05–369–25UG; Villin (anti-mouse)-1:250ul, Santa Cruz, SC-58897; Ki-67 (anti-rabbit)- 1:250ul, Abcam,ab16667; ZO-1, (anti-rabbit)- 1: 250ul, ThermoFisher Scientific, 691–7300; Wnt3 (anti-rabbit)- 1:250ul, Abcam, ab219412; DKK1 (anti-mouse-1:250ul, Santa Cruz, SC-374574; γH2AX (anti-rabbit)-1:250ul, Abcam, ab81299) for overnight at 4°.

    Techniques: Derivative Assay, Cell Culture, Immunohistochemistry, Marker